Posted
Thurs., June 16
Organization
Meyerowitz Lab, Caltech
Position Description
The Meyerowitz lab at Caltech is seeking a research technician to work as a part of a small team to pioneer molecular genetics of a seaweed, Caulerpa.
Responsibilities include:
- Extracting RNA, working with RNA, and maintaining a clean, RNase-free environment on the bench
- Cloning genes, promoters, terminators, and antibiotic resistance genes. Designing, codon-optimizing, and building over-expression constructs using Gibson assembly, Golden-gate assembly, and traditional cloning methods (i.e., using restriction enzymes)
- Performing site-directed mutagenesis when unwanted mutations are detected in your constructs
- Performing transformations of delicate algal specimens using biolistics, carbon nanotubes, injections, or bacterial vectors, and screening for transformants at single micron resolution using confocal microscropy and FLIM microscopy
- Using reverse transcriptase PCR & Western Blot to check for transformation success
- Performing library preparation
- Making stocks and solutions, including artificial seawater
- Maintaining colonies of algae, and responsibly dispose of algal waste
Position Requirements
- Four-year Bachelor of Science or Bachelor of Engineering degree or equivalent experience
- Previous biological wet laboratory experience with molecular biology training
- Previous RNA work and previous success with in situ hybridization a plus
- The ability to build sequencing libraries a plus
- Previous experience with algal genetics and algal molecular biology a plus
Time Commitment
Full-time
Compensation
TBD
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