Organizing lab and projects (maintaining inventory of laboratory supplies, prepare stock reagents, management of lab expenditures)
Maintaining mouse lines (colony maintenance, genotyping, mating, administering treatments to genetically-modified mice to induce esophageal inflammation).
Dissecting mice to isolate tissue-specific protein and mRNA that will require analysis by RT-PCR and western immunoblotting, and isolation of tissue and cells to be set in sections for histopathology and immunofluorescence staining and viewed by bright field and confocal microscopy.
Dealing with culture cells (2D and 3D culture systems) using both mouse and human cell lines and primary cells.
Obtaining informed consent from study participants, attend endoscopy visits to obtain biopsy and peripheral blood samples, and carefully follow study protocols and scripts.
Processing human samples for eosinophilic gastrointestinal diseases biorepository as well as isolating protein and mRNA, and isolation of tissue to be set in sections for histopathology and immunofluorescence staining and viewed by bright field and confocal microscopy.
Completing all necessary paperwork and data documentation, including coding and cleaning data forms, and data entry and tracking.
Maintaining a positive relationship with the study team, clinical staff, and participants, and acts as a study resource.
Working with senior level staff (i.e. administrators, directors, chiefs, clinicians) at research study sites to negotiate/implement/monitor research protocols.
Providing assistance and coordination to various lab research projects.
Attending study team meetings and trainings.
Performing any other related duties as assigned.
Bachelor’s degree in chemistry, biology or physics or a completed animal husbandry program or equivalent in education, training, and experience
At least one year of experience in a laboratory setting (does not include undergraduate research experience) is a plus
Being comfortable with the handling and dissection of laboratory mice as well as working with human biopsy and blood samples is a plus
Experience with REDCap and EPIC is a plus
Full-time, 35 hours per week with a minimum of a two-year commitment and some weekend and evening hours.